Hotspot

petN-psbM

LSC · 29737-30989 · 1253 bp

Back to Celtis

Pi 0.00271
MarkerSeek score 52.20
Primer available yes
Label petN-psbM

Feature Metadata

label_name
petN-psbM
feature_type
igs
region
LSC
length_bp
1253
variable_sites
12
pi
0.00271
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
52.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-psbM_p1 TAGTAAGTCTCGCTTGGGCT TGCTACTGCATTGTTCATTCT 50.0 38.1 58.2 55.9 1414-1420 bp 1.0 54.6
petN-psbM_p2 CACTCGTAGTATGGGGACGA TGCTACTGCATTGTTCATTCT 55.0 38.1 58.3 55.9 1363-1369 bp 1.0 53.3
petN-psbM_p3 TCACTCGTAGTATGGGGACG TGCTACTGCATTGTTCATTCT 55.0 38.1 58.3 55.9 1364-1370 bp 1.0 53.3
petN-psbM_p4 ACTCGTAGTATGGGGACGAA TGCTACTGCATTGTTCATTCT 50.0 38.1 57.6 55.9 1362-1368 bp 1.0 52.5
petN-psbM_p5 TAGTAAGTCTCGCTTGGGCT TGCTACTGCATTGTTCATTC 50.0 40.0 58.2 54.3 1414-1420 bp 1.0 50.0