Hotspot

trnG-UCC-trnR-UCU

LSC · 9905-10248 · 344 bp

Back to Celtis

Pi 0.0148
MarkerSeek score 59.60
Primer available yes
Label trnG-UCC-trnR-UCU

Feature Metadata

label_name
trnG-UCC-trnR-UCU
feature_type
igs
region
LSC
length_bp
344
variable_sites
16
pi
0.0148
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-UCC-trnR-UCU_p1 AGCCTTCCAAGCTAACGATG AGAAGACCTCTGTCCTATCCA 50.0 47.6 58.0 56.9 421-442 bp 1.0 68.0
trnG-UCC-trnR-UCU_p2 CCTAGCCTTCCAAGCTAACG AGAAGACCTCTGTCCTATCCA 55.0 47.6 58.1 56.9 424-445 bp 1.0 67.7
trnG-UCC-trnR-UCU_p3 AGCCTTCCAAGCTAACGATG AGGTTTAGAAGACCTCTGTCCT 50.0 45.5 58.0 57.9 427-448 bp 1.0 67.3
trnG-UCC-trnR-UCU_p4 CCTAGCCTTCCAAGCTAACG AGGTTTAGAAGACCTCTGTCCT 55.0 45.5 58.1 57.9 430-451 bp 1.0 67.0
trnG-UCC-trnR-UCU_p5 ACCAAAGTTTCACGTTCGGT AGAAGACCTCTGTCCTATCCA 45.0 47.6 58.3 56.9 496-517 bp 1.0 65.6