Hotspot

trnH-GUG-psbA

LSC · 75-382 · 308 bp

Back to Celtis

Pi 0.0121
MarkerSeek score 55.50
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
308
variable_sites
11
pi
0.0121
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.96
markerseek_score
55.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 GATCCACTTGGCTACATCCG TCAACCGTGCTAATCTTGGT 55.0 45.0 58.1 57.1 417-430 bp 1.0 77.3
trnH-GUG-psbA_p2 CAATCCACTGCCTTGATCCA TCAACCGTGCTAATCTTGGT 50.0 45.0 57.9 57.1 431-444 bp 1.0 77.1
trnH-GUG-psbA_p3 AATCCACTGCCTTGATCCAC TCAACCGTGCTAATCTTGGT 50.0 45.0 57.9 57.1 430-443 bp 1.0 77.1
trnH-GUG-psbA_p4 ACAATCCACTGCCTTGATCC TCAACCGTGCTAATCTTGGT 50.0 45.0 57.9 57.1 432-445 bp 1.0 77.1
trnH-GUG-psbA_p5 GATCCACTTGGCTACATCCG ACCGTGCTAATCTTGGTATGG 55.0 47.6 58.1 57.8 414-427 bp 1.0 74.4