Hotspot

ndhF-rpl32

SSC · 112783-113860 · 1078 bp

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Pi 0.00371

MarkerSeek score 61.00

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1078
variable_sites: 4
pi: 0.00371
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GCCGCTGGGAAAAAGAAAAG	TTAATGCTGCCCAATACCCC	50.0	50.0	58.2	57.9	1261-1269 bp	1.0	77.9
ndhF-rpl32_p2	GCCGCTGGGAAAAAGAAAAG	AAGGCTTTTAATGCTGCCCA	50.0	45.0	58.2	58.4	1268-1276 bp	1.0	75.7
ndhF-rpl32_p3	GCCGCTGGGAAAAAGAAAAG	AGGCTTTTAATGCTGCCCAA	50.0	45.0	58.2	58.4	1267-1275 bp	1.0	75.7
ndhF-rpl32_p4	GCCGCTGGGAAAAAGAAAAG	GGCTTTTAATGCTGCCCAAT	50.0	45.0	58.2	57.3	1266-1274 bp	1.0	73.0
ndhF-rpl32_p5	GCCGCTGGGAAAAAGAAAAG	GCTGCCCAATACCCCTTTTT	50.0	50.0	58.2	58.7	1256-1264 bp	1.0	72.8