Hotspot

psbE-petL

LSC · 65169-66512 · 1344 bp

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Pi 0.0112

MarkerSeek score 53.90

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1344
variable_sites: 15
pi: 0.0112
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	TGACAAATAACCAACCCGCA	CCGAAATAACTAGTTAGAGTAGGCA	45.0	40.0	58.0	58.0	1485-1489 bp	1.0	40.9
psbE-petL_p2	TGACAAATAACCAACCCGCA	ACCGAAATAACTAGTTAGAGTAGGC	45.0	40.0	58.0	58.0	1486-1490 bp	1.0	40.9
psbE-petL_p3	CAACCCGCAATGAATAGGGA	CCGAAATAACTAGTTAGAGTAGGCA	50.0	40.0	57.9	58.0	1474-1478 bp	1.0	40.9
psbE-petL_p4	CAACCCGCAATGAATAGGGA	ACCGAAATAACTAGTTAGAGTAGGC	50.0	40.0	57.9	58.0	1475-1479 bp	1.0	40.9
psbE-petL_p5	AGAACGTTCTCCTGTGCTTC	CCGAAATAACTAGTTAGAGTAGGCA	50.0	40.0	57.8	58.0	1397-1401 bp	1.0	40.9