Hotspot

petA-psbJ

LSC · 63467-64367 · 901 bp

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Pi 0.0191

MarkerSeek score 71.10

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 901
variable_sites: 17
pi: 0.0191
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 71.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CACTGCGTGTACAAGGTCTT	ACTGGTATTGTTGTGATCGGT	50.0	42.9	58.1	57.3	1091-1092 bp	1.0	67.6
petA-psbJ_p2	CACTGCGTGTACAAGGTCTT	GCCGATACTACTGGAAGGAT	50.0	50.0	58.1	56.2	1136-1137 bp	1.0	66.7
petA-psbJ_p3	CACTGCGTGTACAAGGTCTT	CTGGATTAGGCTCATCACTGT	50.0	47.6	58.1	57.2	1039-1040 bp	1.0	66.6
petA-psbJ_p4	CACTGCGTGTACAAGGTCTT	AACTGGTATTGTTGTGATCGGT	50.0	40.9	58.1	58.0	1092-1093 bp	1.0	65.0
petA-psbJ_p5	CACTGCGTGTACAAGGTCTT	ACTGGTATTGTTGTGATCGGTT	50.0	40.9	58.1	58.0	1091-1092 bp	1.0	65.0