Hotspot

ndhC-trnV-UAC

LSC · 51583-52294 · 712 bp

Back to Celosia

Pi 0.00707

MarkerSeek score 63.50

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 712
variable_sites: 5
pi: 0.00707
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	CTCGGGTCCTTTGTTACTCG	AGAAGGTCTACGGTTCGAGT	55.0	50.0	58.0	57.8	862-875 bp	1.0	79.3
ndhC-trnV-UAC_p2	GGTCCTTTGTTACTCGGAGC	AGAAGGTCTACGGTTCGAGT	55.0	50.0	58.3	57.8	858-871 bp	1.0	77.1
ndhC-trnV-UAC_p3	CTCGGGTCCTTTGTTACTCG	GAAGGTCTACGGTTCGAGTC	55.0	55.0	58.0	57.5	861-874 bp	1.0	76.5
ndhC-trnV-UAC_p4	GGGTCCTTTGTTACTCGGAG	AGAAGGTCTACGGTTCGAGT	55.0	50.0	57.6	57.8	859-872 bp	1.0	76.3
ndhC-trnV-UAC_p5	CTCGGGTCCTTTGTTACTCG	GTTCGAGTCCGTATAGCCCT	55.0	55.0	58.0	58.7	850-863 bp	1.0	75.4