Hotspot

psbC-trnS-UGA

LSC · 36173-36413 · 241 bp

Back to Celosia

Pi 0.029

MarkerSeek score 60.30

Primer available yes

Label psbC-trnS-UGA

Feature Metadata

label_name: psbC-trnS-UGA
feature_type: igs
region: LSC
length_bp: 241
variable_sites: 7
pi: 0.029
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS-UGA_p1	GCGGCAGGATTTGAAAAAGG	GGTTGATAGCTCCGGTCTTG	50.0	55.0	58.3	58.1	387 bp	1.0	78.7
psbC-trnS-UGA_p2	GCGGCAGGATTTGAAAAAGG	GGGTTCGAATCCCTCTCTCT	50.0	55.0	58.3	58.2	333 bp	1.0	77.7
psbC-trnS-UGA_p3	CTGCGGCAGGATTTGAAAAA	GGTTGATAGCTCCGGTCTTG	45.0	55.0	57.6	58.1	389 bp	1.0	77.5
psbC-trnS-UGA_p4	TGCGGCAGGATTTGAAAAAG	GGTTGATAGCTCCGGTCTTG	45.0	55.0	57.6	58.1	388 bp	1.0	77.5
psbC-trnS-UGA_p5	TGCGGCAGGATTTGAAAAAG	GGGTTCGAATCCCTCTCTCT	45.0	55.0	57.6	58.2	334 bp	1.0	76.4