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trnC-GCA-petN

LSC · 28477-28973 · 497 bp

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Pi 0.08

MarkerSeek score 81.20

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 497
variable_sites: 32
pi: 0.08
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.80
markerseek_score: 81.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TGTTCCCCAGTTCAAATCCG	TTCTAGAGCCCACTTCTCCC	50.0	55.0	58.1	58.1	619-641 bp	1.0	86.2
trnC-GCA-petN_p2	TGTTCCCCAGTTCAAATCCG	GCCCAAGCGAGACTTACAAT	50.0	50.0	58.1	58.3	553-575 bp	1.0	85.7
trnC-GCA-petN_p3	GCAAATCCTTGTTCCCCAGT	TTCTAGAGCCCACTTCTCCC	50.0	55.0	58.4	58.1	628-650 bp	1.0	84.0
trnC-GCA-petN_p4	CTGCAAATCCTTGTTCCCCA	TTCTAGAGCCCACTTCTCCC	50.0	55.0	58.4	58.1	630-652 bp	1.0	84.0
trnC-GCA-petN_p5	TGCAAATCCTTGTTCCCCAG	TTCTAGAGCCCACTTCTCCC	50.0	55.0	58.4	58.1	629-651 bp	1.0	84.0