Hotspot

trnK-UUU-rps16

LSC · 4377-5303 · 927 bp

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Pi 0.00444
MarkerSeek score 63.70
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
927
variable_sites
4
pi
0.00444
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
63.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT GCTCAACCCACAGAAACTGT 50.0 50.0 58.2 58.3 1018-1042 bp 1.0 78.4
trnK-UUU-rps16_p2 AAAGCCGAGTACTCTACCGT GTGCTCAACCCACAGAAACT 50.0 50.0 58.2 58.3 1020-1044 bp 1.0 78.4
trnK-UUU-rps16_p3 AAGCCGAGTACTCTACCGTT GCTCAACCCACAGAAACTGT 50.0 50.0 58.2 58.3 1017-1041 bp 1.0 78.4
trnK-UUU-rps16_p4 AAGCCGAGTACTCTACCGTT GTGCTCAACCCACAGAAACT 50.0 50.0 58.2 58.3 1019-1043 bp 1.0 78.4
trnK-UUU-rps16_p5 AAGCCGAGTACTCTACCGTT TGCTCAACCCACAGAAACTG 50.0 50.0 58.2 58.3 1018-1042 bp 1.0 78.4