Hotspot

trnH-GUG-psbA

LSC · 72-507 · 436 bp

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Pi 0.0171
MarkerSeek score 69.60
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
436
variable_sites
7
pi
0.0171
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.94
markerseek_score
69.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 GATCCACTTGGCTACATCCG GCTATCGAAGCCCCATCTAC 55.0 55.0 58.1 57.6 473-487 bp 1.0 81.2
trnH-GUG-psbA_p2 GATCCACTTGGCTACATCCG CCGTGCTAACCTTGGTATGG 55.0 55.0 58.1 58.3 540-554 bp 1.0 81.1
trnH-GUG-psbA_p3 CAATCCACTGCCTTGATCCA CCGTGCTAACCTTGGTATGG 50.0 55.0 57.9 58.3 554-568 bp 1.0 80.9
trnH-GUG-psbA_p4 AATCCACTGCCTTGATCCAC CCGTGCTAACCTTGGTATGG 50.0 55.0 57.9 58.3 553-567 bp 1.0 80.9
trnH-GUG-psbA_p5 ACAATCCACTGCCTTGATCC CCGTGCTAACCTTGGTATGG 50.0 55.0 57.9 58.3 555-569 bp 1.0 80.9