Hotspot

ycf1

SSC · 124839-130394 · 5556 bp

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Pi 0.0148

MarkerSeek score 52.30

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5556
variable_sites: 320
pi: 0.0148
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GGTATGGCTCTGGGGTTTTT	ACAAATTCTTCACTGGCCGA	50.0	45.0	57.8	57.7	1448-1472 bp	1.0	82.3
ycf1_p2	CACAATCGCATCTTCTCCCA	ACAAATTCTTCACTGGCCGA	50.0	45.0	58.0	57.7	1095-1110 bp	0.833333	76.1
ycf1_p3	TCCACAATCGCATCTTCTCC	ACAAATTCTTCACTGGCCGA	50.0	45.0	57.7	57.7	1097-1112 bp	0.833333	73.8
ycf1_p4	ACAATCGCATCTTCTCCCAG	ACAAATTCTTCACTGGCCGA	50.0	45.0	57.7	57.7	1094-1109 bp	0.833333	73.7
ycf1_p5	CACAATCGCATCTTCTCCCA	AAAATCACGAAAACGCTGCT	50.0	40.0	58.0	57.2	749-764 bp	0.666667	65.5