Hotspot

rpl16

LSC · 81794-83298 · 1505 bp

Back to Celastrus

Pi 0.0139

MarkerSeek score 58.20

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1505
variable_sites: 76
pi: 0.0139
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 58.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	ACAGCCAAAAGGCGAAGTAA	CAAGTACAAATTGCAGGGCG	45.0	50.0	58.0	58.0	1964-2005 bp	1.0	87.6
rpl16_p2	ACAGCCAAAAGGCGAAGTAA	GCAGAATTTCTAGCCGGACA	45.0	50.0	58.0	58.0	2063-2104 bp	1.0	87.5
rpl16_p3	TGTCAGCAAGAGTGTCCCTA	CAAGTACAAATTGCAGGGCG	50.0	50.0	58.0	58.0	2551-2596 bp	1.0	87.1
rpl16_p4	TGTCAGCAAGAGTGTCCCTA	GCAGAATTTCTAGCCGGACA	50.0	50.0	58.0	58.0	2650-2695 bp	1.0	86.9
rpl16_p5	TGTCAGCAAGAGTGTCCCTA	TGCAGAATTTCTAGCCGGAC	50.0	50.0	58.0	58.0	2651-2696 bp	1.0	86.9