Hotspot

ccsA-ndhD

SSC · 116198-116448 · 251 bp

Back to Celastrus

Pi 0.0312

MarkerSeek score 65.70

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 251
variable_sites: 26
pi: 0.0312
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	AGGAATAGGGCTCCACAGTT	GGCATTTATCCGGACTTCGT	50.0	50.0	58.0	58.1	369-377 bp	1.0	87.1
ccsA-ndhD_p2	AGGAATAGGGCTCCACAGTT	TGGCATTTATCCGGACTTCG	50.0	50.0	58.0	58.1	370-378 bp	1.0	87.1
ccsA-ndhD_p3	GGGCTCCACAGTTATGGTTC	GGCATTTATCCGGACTTCGT	55.0	50.0	58.2	58.1	362-370 bp	1.0	85.5
ccsA-ndhD_p4	GGGCTCCACAGTTATGGTTC	TGGCATTTATCCGGACTTCG	55.0	50.0	58.2	58.1	363-371 bp	1.0	85.5
ccsA-ndhD_p5	TAGGGCTCCACAGTTATGGT	GGCATTTATCCGGACTTCGT	50.0	50.0	57.7	58.1	364-372 bp	1.0	85.3