Hotspot

rpl22-rps19

LSC · 84671-84751 · 81 bp

Back to Celastrus

Pi 0.0389

MarkerSeek score 67.40

Primer available yes

Label rpl22-rps19

Feature Metadata

label_name: rpl22-rps19
feature_type: igs
region: LSC
length_bp: 81
variable_sites: 9
pi: 0.0389
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 67.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22-rps19_p1	CTACCCTCGTTGACTTCAGC	AGCTTAACACAAAGGCGGAA	55.0	45.0	58.0	58.0	554-589 bp	1.0	87.5
rpl22-rps19_p2	CTACCCTCGTTGACTTCAGC	AACAGTCGGACAAGTAGGGA	55.0	50.0	58.0	58.0	934-969 bp	1.0	86.2
rpl22-rps19_p3	CTACCCTCGTTGACTTCAGC	ACAGTCGGACAAGTAGGGAA	55.0	50.0	58.0	58.0	933-968 bp	1.0	86.2
rpl22-rps19_p4	TTGTCCCATAATCCCCCACT	AACAGTCGGACAAGTAGGGA	50.0	50.0	58.0	58.0	1192-1226 bp	1.0	85.8
rpl22-rps19_p5	TTGTCCCATAATCCCCCACT	ACAGTCGGACAAGTAGGGAA	50.0	50.0	58.0	58.0	1191-1225 bp	1.0	85.8