Hotspot

clpP-psbB

LSC · 72532-72976 · 445 bp

Back to Celastrus

Pi 0.00979

MarkerSeek score 67.10

Primer available yes

Label clpP-psbB

Feature Metadata

label_name: clpP-psbB
feature_type: igs
region: LSC
length_bp: 445
variable_sites: 24
pi: 0.00979
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 67.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP-psbB_p1	AAGCGTTTTCCCCAGGATTT	CGATACCAAGGCAAACCCAT	45.0	50.0	58.0	58.2	503-517 bp	0.916667	81.3
clpP-psbB_p2	AACCCAGGATGAAGCGTTTT	CGATACCAAGGCAAACCCAT	45.0	50.0	58.0	58.2	514-528 bp	0.916667	81.2
clpP-psbB_p3	AACCCAGGATGAAGCGTTTT	CAAACGACCGGGATCATTCA	45.0	50.0	58.0	58.3	551-565 bp	0.916667	80.6
clpP-psbB_p4	AAGCGTTTTCCCCAGGATTT	CAAACGACCGGGATCATTCA	45.0	50.0	58.0	58.3	540-554 bp	0.916667	80.6
clpP-psbB_p5	ATGTCAACCCAGGATGAAGC	CGATACCAAGGCAAACCCAT	50.0	50.0	57.9	58.2	519-533 bp	0.916667	80.1