Hotspot

trnM-CAU-atpE

LSC · 53175-53455 · 281 bp

Back to Celastrus

Pi 0.0416

MarkerSeek score 72.10

Primer available yes

Label trnM-CAU-atpE

Feature Metadata

label_name: trnM-CAU-atpE
feature_type: igs
region: LSC
length_bp: 281
variable_sites: 28
pi: 0.0416
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 72.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnM-CAU-atpE_p1	TATTGCTTTCATACGGCGGG	CTCTCAGACGAGCTAGGACA	50.0	55.0	58.4	58.0	239-400 bp	1.0	86.5
trnM-CAU-atpE_p2	TATTGCTTTCATACGGCGGG	TCTCAGACGAGCTAGGACAC	50.0	55.0	58.4	58.3	238-399 bp	1.0	84.7
trnM-CAU-atpE_p3	GTATTGCTTTCATACGGCGG	CTCTCAGACGAGCTAGGACA	50.0	55.0	57.3	58.0	240-401 bp	1.0	84.5
trnM-CAU-atpE_p4	GCGGGAGTCATTGGTTCAAA	CTCTCAGACGAGCTAGGACA	50.0	55.0	58.8	58.0	224-385 bp	1.0	84.0
trnM-CAU-atpE_p5	TATTGCTTTCATACGGCGGG	GGAGGCTATCAATGGGATGG	50.0	55.0	58.4	57.5	214-375 bp	1.0	83.1