Hotspot

trnF-GAA-ndhJ

LSC · 49218-50007 · 790 bp

Back to Celastrus

Pi 0.0195

MarkerSeek score 72.90

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 790
variable_sites: 63
pi: 0.0195
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 72.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	CCCGAAAGTTGGATAGGGTG	55.0	55.0	58.0	58.0	938-968 bp	1.0	86.5
trnF-GAA-ndhJ_p2	GGGATAGCTCAGCTGGTAGA	CCGAAAGTTGGATAGGGTGG	55.0	55.0	58.0	58.0	937-967 bp	1.0	86.5
trnF-GAA-ndhJ_p3	CCTCGTGTCACCAGTTCAAA	CCCGAAAGTTGGATAGGGTG	50.0	55.0	58.1	58.0	902-932 bp	1.0	86.4
trnF-GAA-ndhJ_p4	CCTCGTGTCACCAGTTCAAA	CCGAAAGTTGGATAGGGTGG	50.0	55.0	58.1	58.0	901-931 bp	1.0	86.4
trnF-GAA-ndhJ_p5	TATGCGTTGAGAATGGTCGG	CCGAAAGTTGGATAGGGTGG	50.0	55.0	58.1	58.0	955-985 bp	1.0	86.0