Hotspot

psbZ-trnG-GCC

LSC · 36403-37126 · 724 bp

Back to Celastrus

Pi 0.0253

MarkerSeek score 67.00

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 724
variable_sites: 66
pi: 0.0253
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 67.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	AGCACGACCTTTTGTGAAGT	CTTGAGGTCACGGGTTCAAA	45.0	50.0	58.0	58.0	1250-1458 bp	1.0	89.1
psbZ-trnG-GCC_p2	CAAGACCGGAGCTATCAACC	CTTGAGGTCACGGGTTCAAA	55.0	50.0	58.1	58.0	1454-1661 bp	1.0	89.0
psbZ-trnG-GCC_p3	AGCACGACCTTTTGTGAAGT	CGACGTTGTTTTTCGACTGG	45.0	50.0	58.0	58.0	1575-1783 bp	1.0	88.8
psbZ-trnG-GCC_p4	CAAGACCGGAGCTATCAACC	CGACGTTGTTTTTCGACTGG	55.0	50.0	58.1	58.0	1779-1986 bp	1.0	88.8
psbZ-trnG-GCC_p5	ATTCGCCAAGCAAAAGGAGA	CGACGTTGTTTTTCGACTGG	45.0	50.0	58.1	58.0	1850-2057 bp	1.0	88.4