Hotspot

psbM-trnD-GUC

LSC · 29802-30427 · 626 bp

Back to Celastrus

Pi 0.0143

MarkerSeek score 70.60

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 626
variable_sites: 35
pi: 0.0143
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	CGCCCTGACTTTGACTGATA	TTCAATTGGTCAGAGCACCG	50.0	50.0	57.3	58.5	633-810 bp	1.0	78.5
psbM-trnD-GUC_p2	TATCGCCCTGACTTTGACTG	TTCAATTGGTCAGAGCACCG	50.0	50.0	57.3	58.5	636-813 bp	1.0	78.5
psbM-trnD-GUC_p3	ATCGCCCTGACTTTGACTGA	TTCAATTGGTCAGAGCACCG	50.0	50.0	59.0	58.5	635-812 bp	1.0	75.7
psbM-trnD-GUC_p4	TCGCCCTGACTTTGACTGAT	TTCAATTGGTCAGAGCACCG	50.0	50.0	59.0	58.5	634-811 bp	1.0	75.7
psbM-trnD-GUC_p5	TTATCGCCCTGACTTTGACTG	TTCAATTGGTCAGAGCACCG	47.6	50.0	58.0	58.5	637-814 bp	1.0	75.6