Hotspot

atpA-atpF

LSC · 11479-11543 · 65 bp

Back to Celastrus

Pi 0.0806

MarkerSeek score 74.20

Primer available yes

Label atpA-atpF

Feature Metadata

label_name: atpA-atpF
feature_type: igs
region: LSC
length_bp: 65
variable_sites: 13
pi: 0.0806
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 74.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA-atpF_p1	ACTAATTTCGTCGGCTCGAA	GGCATCTTTGGGACGATGAA	45.0	50.0	57.4	58.2	124-136 bp	1.0	84.9
atpA-atpF_p2	ACTAATTTCGTCGGCTCGAA	ATTGGCATCTTTGGGACGAT	45.0	45.0	57.4	57.6	127-139 bp	1.0	83.3
atpA-atpF_p3	ACTAATTTCGTCGGCTCGAA	TTGGCATCTTTGGGACGATG	45.0	50.0	57.4	58.5	126-138 bp	1.0	82.6
atpA-atpF_p4	ACTAATTTCGTCGGCTCGAA	ACAAGCCCTACAAGAAGCTC	45.0	50.0	57.4	57.5	206-218 bp	1.0	82.3
atpA-atpF_p5	AATTTCGTCGGCTCGAATGG	GGCATCTTTGGGACGATGAA	50.0	50.0	59.0	58.2	121-133 bp	1.0	82.1