Hotspot

trnG-UCC-trnR-UCU

LSC · 9265-9632 · 368 bp

Back to Celastrus

Pi 0.0411

MarkerSeek score 69.60

Primer available yes

Label trnG-UCC-trnR-UCU

Feature Metadata

label_name: trnG-UCC-trnR-UCU
feature_type: igs
region: LSC
length_bp: 368
variable_sites: 47
pi: 0.0411
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 69.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnR-UCU_p1	AGCCTTCCAAGCTAACGATG	TGTCCTATCCATTAGACAATG	50.0	38.1	58.0	52.4	413-465 bp	1.0	52.0
trnG-UCC-trnR-UCU_p2	CCTAGCCTTCCAAGCTAACG	TGTCCTATCCATTAGACAATG	55.0	38.1	58.1	52.4	416-468 bp	1.0	52.0
trnG-UCC-trnR-UCU_p3	AACGCCGACTATAACCCCTA	TGTCCTATCCATTAGACAATG	50.0	38.1	57.9	52.4	432-484 bp	1.0	52.0
trnG-UCC-trnR-UCU_p4	CCGACTATAACCCCTAGCCT	TGTCCTATCCATTAGACAATG	55.0	38.1	57.7	52.4	428-480 bp	1.0	52.0
trnG-UCC-trnR-UCU_p5	CCCTAGCCTTCCAAGCTAAC	TGTCCTATCCATTAGACAATG	55.0	38.1	57.7	52.4	417-469 bp	1.0	52.0