Hotspot

rps15

SSC · 117905-118177 · 273 bp

Back to Cedrela

Pi 0.49

MarkerSeek score 75.50

Primer available yes

Label rps15

Feature Metadata

label_name: rps15
feature_type: gene
region: SSC
length_bp: 273
variable_sites: 118
pi: 0.49
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 75.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15_p1	TGGCAGTCGTTTCAGTATGG	GAAAGGAGTCTGTGCACCAA	50.0	50.0	57.9	58.0	1209 bp	0.5	59.0
rps15_p2	GGCAGTCGTTTCAGTATGGT	GAAAGGAGTCTGTGCACCAA	50.0	50.0	57.9	58.0	1208 bp	0.5	59.0
rps15_p3	TGGCAGTCGTTTCAGTATGG	CATGAGGTAAATCAGCCGCT	50.0	50.0	57.9	58.0	1324 bp	0.5	58.9
rps15_p4	GGCAGTCGTTTCAGTATGGT	CATGAGGTAAATCAGCCGCT	50.0	50.0	57.9	58.0	1323 bp	0.5	58.9
rps15_p5	TGGCAGTCGTTTCAGTATGG	TCTGTGCACCAATATCTGCC	50.0	50.0	57.9	58.0	1201 bp	0.5	58.9