Hotspot

rpl32

SSC · 128240-128398 · 159 bp

Back to Cedrela

Pi 0.482

MarkerSeek score 75.70

Primer available yes

Label rpl32

Feature Metadata

label_name: rpl32
feature_type: gene
region: SSC
length_bp: 159
variable_sites: 68
pi: 0.482
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 75.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32_p1	ATGCAAGTGCGAGATATGGG	AGGTACCACCAATAACTCGGA	50.0	47.6	58.1	58.2	899 bp	0.5	49.7
rpl32_p2	ATTGGAATGCAAGTGCGAGA	AGGTACCACCAATAACTCGGA	45.0	47.6	58.2	58.2	905 bp	0.5	49.2
rpl32_p3	TTGGAATGCAAGTGCGAGAT	AGGTACCACCAATAACTCGGA	45.0	47.6	58.2	58.2	904 bp	0.5	49.2
rpl32_p4	GCAAGTGCGAGATATGGGAT	AGGTACCACCAATAACTCGGA	50.0	47.6	57.8	58.2	897 bp	0.5	49.2
rpl32_p5	TGCCTAAATGGGAAAGGGTG	AGGTACCACCAATAACTCGGA	50.0	47.6	57.8	58.2	934 bp	0.5	48.8