Hotspot

ndhD

SSC · 124235-125761 · 1527 bp

Back to Cedrela

Pi 0.494

MarkerSeek score 81.80

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1527
variable_sites: 671
pi: 0.494
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 81.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	CAAATTGCTTCGGCTCCAAG	AAAGGCGTCTGGTCACAATT	50.0	45.0	58.0	58.0	1969 bp	0.5	59.7
ndhD_p2	AAACAACTCGAAGCATGGGT	AAAGGCGTCTGGTCACAATT	45.0	45.0	58.0	58.0	1866 bp	0.5	59.7
ndhD_p3	GGCATGAAACAACTCGAAGC	AAAGGCGTCTGGTCACAATT	50.0	45.0	58.0	58.0	1872 bp	0.5	59.6
ndhD_p4	ACCGATTACCCACAAAACCC	AAAGGCGTCTGGTCACAATT	50.0	45.0	58.1	58.0	1775 bp	0.5	59.2
ndhD_p5	GATTACCCACAAAACCCCGT	AAAGGCGTCTGGTCACAATT	50.0	45.0	58.1	58.0	1772 bp	0.5	59.2