Hotspot

ndhA

SSC · 119478-121662 · 2185 bp

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Pi 0.478

MarkerSeek score 79.40

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2185
variable_sites: 888
pi: 0.478
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.85
markerseek_score: 79.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	TCGGTGAAATGACGGAATCC	GACAGACGCGAACACATACT	50.0	50.0	58.0	58.0	2879 bp	0.5	59.7
ndhA_p2	AGCCCCAAAAGGAGAATTGG	GACAGACGCGAACACATACT	50.0	50.0	58.1	58.0	2680 bp	0.5	59.4
ndhA_p3	AAGAGTCGAAGCCCCAAAAG	GACAGACGCGAACACATACT	50.0	50.0	58.1	58.0	2689 bp	0.5	59.0
ndhA_p4	TCCGACTTTTGAATTGCCGA	GACAGACGCGAACACATACT	45.0	50.0	58.1	58.0	2725 bp	0.5	58.9
ndhA_p5	ATGGGGGAAGTTGATCGTTG	GACAGACGCGAACACATACT	50.0	50.0	57.9	58.0	2511 bp	0.5	58.8