Hotspot

rpl32-ndhF

SSC · 128399-129385 · 987 bp

Back to Cedrela

Pi 0.434

MarkerSeek score 82.30

Primer available yes

Label rpl32-ndhF

Feature Metadata

label_name: rpl32-ndhF
feature_type: igs
region: SSC
length_bp: 987
variable_sites: 388
pi: 0.434
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 82.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-ndhF_p1	ATGCAAGTGCGAGATATGGG	AACAGAAGCCCCACTCCTAT	50.0	50.0	58.1	58.0	1698 bp	0.5	58.9
rpl32-ndhF_p2	ATGCAAGTGCGAGATATGGG	ACAGAAGCCCCACTCCTATT	50.0	50.0	58.1	58.0	1697 bp	0.5	58.9
rpl32-ndhF_p3	ATTGGAATGCAAGTGCGAGA	ACAGAAGCCCCACTCCTATT	45.0	50.0	58.2	58.0	1703 bp	0.5	58.4
rpl32-ndhF_p4	TTGGAATGCAAGTGCGAGAT	AACAGAAGCCCCACTCCTAT	45.0	50.0	58.2	58.0	1703 bp	0.5	58.4
rpl32-ndhF_p5	TTGGAATGCAAGTGCGAGAT	ACAGAAGCCCCACTCCTATT	45.0	50.0	58.2	58.0	1702 bp	0.5	58.4