Hotspot

trnL-UAG-rpl32

SSC · 127214-128239 · 1026 bp

Back to Cedrela

Pi 0.44

MarkerSeek score 79.90

Primer available yes

Label trnL-UAG-rpl32

Feature Metadata

label_name: trnL-UAG-rpl32
feature_type: igs
region: SSC
length_bp: 1026
variable_sites: 370
pi: 0.44
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.82
markerseek_score: 79.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAG-rpl32_p1	AGCGTGTCTACCGATTTCAC	CGAAATCCCTTTCTACCGGG	50.0	55.0	58.0	58.1	1111 bp	0.5	59.5
trnL-UAG-rpl32_p2	GAGCGGGTACTAATATGCCG	CGAAATCCCTTTCTACCGGG	55.0	55.0	58.0	58.1	1655 bp	0.5	59.5
trnL-UAG-rpl32_p3	CAGCGTGTCTACCGATTTCA	CGAAATCCCTTTCTACCGGG	50.0	55.0	58.0	58.1	1112 bp	0.5	59.5
trnL-UAG-rpl32_p4	GAAGTAGCAAAGCCTTGGGT	CGAAATCCCTTTCTACCGGG	50.0	55.0	58.1	58.1	1608 bp	0.5	58.9
trnL-UAG-rpl32_p5	ATTCATTGGCGGGCTTTTTG	CGAAATCCCTTTCTACCGGG	45.0	55.0	57.9	58.1	1203 bp	0.5	58.8