Hotspot

psaC-ndhD

SSC · 124098-124234 · 137 bp

Back to Cedrela

Pi 0.508

MarkerSeek score 76.60

Primer available yes

Label psaC-ndhD

Feature Metadata

label_name: psaC-ndhD
feature_type: igs
region: SSC
length_bp: 137
variable_sites: 64
pi: 0.508
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 76.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhD_p1	CAAATTGCTTCGGCTCCAAG	TTCCCAATCGCCAGTCAAAA	50.0	45.0	58.0	58.0	543 bp	0.5	59.8
psaC-ndhD_p2	AAACAACTCGAAGCATGGGT	TTCCCAATCGCCAGTCAAAA	45.0	45.0	58.0	58.0	440 bp	0.5	59.8
psaC-ndhD_p3	CAAATTGCTTCGGCTCCAAG	AAGACGTTTCTTTCCACCCC	50.0	50.0	58.0	58.0	782 bp	0.5	59.7
psaC-ndhD_p4	AAACAACTCGAAGCATGGGT	AAGACGTTTCTTTCCACCCC	45.0	50.0	58.0	58.0	679 bp	0.5	59.7
psaC-ndhD_p5	GGCATGAAACAACTCGAAGC	TTCCCAATCGCCAGTCAAAA	50.0	45.0	58.0	58.0	446 bp	0.5	59.7