Hotspot

rpl16

LSC · 86034-87538 · 1505 bp

Back to Cautleya

Pi 0.0198

MarkerSeek score 60.20

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1505
variable_sites: 29
pi: 0.0198
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	ACACATCAATTCTCGGGCTC	GGTTTCGAAGGAATTGCACG	50.0	50.0	58.0	58.0	2320-2332 bp	1.0	82.1
rpl16_p2	ACACATCAATTCTCGGGCTC	GTTTCGAAGGAATTGCACGG	50.0	50.0	58.0	58.0	2319-2331 bp	1.0	82.1
rpl16_p3	ACACATCAATTCTCGGGCTC	GGACGGATCAACGGAAAAGA	50.0	50.0	58.0	57.9	1990-2002 bp	1.0	82.1
rpl16_p4	GTATATGCGCGAGACACCAT	GGTTTCGAAGGAATTGCACG	50.0	50.0	57.9	58.0	2741-2753 bp	1.0	81.4
rpl16_p5	GTATATGCGCGAGACACCAT	GTTTCGAAGGAATTGCACGG	50.0	50.0	57.9	58.0	2740-2752 bp	1.0	81.4