Hotspot

psbH

LSC · 79204-79425 · 222 bp

Back to Cautleya

Pi 0.0045

MarkerSeek score 41.40

Primer available yes

Label psbH

Feature Metadata

label_name: psbH
feature_type: gene
region: LSC
length_bp: 222
variable_sites: 1
pi: 0.0045
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 41.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbH_p1	TTGCTCTGGTATCTTTCGCC	GCCAAGCTAAACTCAAGGGT	50.0	50.0	58.0	58.1	1319-1345 bp	1.0	80.1
psbH_p2	TCAACTAGTCCCCGTGTTCT	GCCAAGCTAAACTCAAGGGT	50.0	50.0	58.0	58.1	983-1003 bp	1.0	79.9
psbH_p3	CCCGTGTTCTTCGAATGGAT	GCCAAGCTAAACTCAAGGGT	50.0	50.0	58.0	58.1	973-993 bp	1.0	79.7
psbH_p4	TCAACTAGTCCCCGTGTTCT	TTTTGGAGTCCGGTTTCGAG	50.0	50.0	58.0	58.1	805 bp	0.5	58.9
psbH_p5	CCCGTGTTCTTCGAATGGAT	TTTTGGAGTCCGGTTTCGAG	50.0	50.0	58.0	58.1	795 bp	0.5	58.8