Hotspot

ndhB

IRb · 100095-102327 · 2233 bp

Back to Cautleya

Pi 0.0119

MarkerSeek score 65.90

Primer available yes

Label ndhB

Feature Metadata

label_name: ndhB
feature_type: gene
region: IRb
length_bp: 2233
variable_sites: 26
pi: 0.0119
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 65.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhB_p1	GTTCATAACCCCAGCCCATT	TTCGGAGATTGGATGCAGTT	50.0	45.0	57.8	57.2	2363 bp	1.0	74.8
ndhB_p2	GTTCATAACCCCAGCCCATT	TTTCGGAGATTGGATGCAGT	50.0	45.0	57.8	57.2	2364 bp	1.0	74.8
ndhB_p3	TTTGGGCGGAACAGATCTAC	TTCGGAGATTGGATGCAGTT	50.0	45.0	57.6	57.2	2338 bp	1.0	72.9
ndhB_p4	TTTGGGCGGAACAGATCTAC	TTTCGGAGATTGGATGCAGT	50.0	45.0	57.6	57.2	2339 bp	1.0	72.9
ndhB_p5	TTGGGCGGAACAGATCTACT	TTCGGAGATTGGATGCAGTT	50.0	45.0	58.4	57.2	2337 bp	1.0	72.6