Hotspot

rps15-ycf1

SSC · 132637-133078 · 442 bp

Back to Cautleya

Pi 0.00487

MarkerSeek score 62.50

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 442
variable_sites: 2
pi: 0.00487
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 62.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	ACTTGAAATTCAACAGAACCTCT	TGGCCCAATTACCGATTAGA	34.8	45.0	56.3	56.3	614-639 bp	1.0	44.3
rps15-ycf1_p2	ACTTGAAATTCAACAGAACCTCT	TGGCCCAATTACCGATTAGAA	34.8	42.9	56.3	57.1	614-639 bp	1.0	44.3
rps15-ycf1_p3	ACTTGAAATTCAACAGAACCTCT	GGCCCAATTACCGATTAGAAGA	34.8	45.5	56.3	57.9	613-638 bp	1.0	44.3
rps15-ycf1_p4	ACTTGAAATTCAACAGAACCTC	TGGCCCAATTACCGATTAGA	36.4	45.0	54.9	56.3	614-639 bp	1.0	44.3
rps15-ycf1_p5	ACTTGAAATTCAACAGAACCTCT	TGGCCCAATTACCGATTAGAAG	34.8	45.5	56.3	58.2	614-639 bp	1.0	44.3