Hotspot

trnL-UAG-ccsA

SSC · 122719-122746 · 28 bp

Back to Cautleya

Pi 0.0357

MarkerSeek score 73.00

Primer available yes

Label trnL-UAG-ccsA

Feature Metadata

label_name: trnL-UAG-ccsA
feature_type: igs
region: SSC
length_bp: 28
variable_sites: 1
pi: 0.0357
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAG-ccsA_p1	TAGACACGCTGCTCTTAGGA	AGGAGTAGGATCCATTGTGTCT	50.0	45.5	57.9	58.0	113 bp	1.0	63.7
trnL-UAG-ccsA_p2	TAAGCCGCCATGGTGAAATT	AGGAGTAGGATCCATTGTGTCT	45.0	45.5	58.2	58.0	135 bp	1.0	63.4
trnL-UAG-ccsA_p3	AAATTGGTAGACACGCTGCT	AGGAGTAGGATCCATTGTGTCT	45.0	45.5	57.8	58.0	120 bp	1.0	63.2
trnL-UAG-ccsA_p4	GGTAGACACGCTGCTCTTAG	AGGAGTAGGATCCATTGTGTCT	55.0	45.5	57.8	58.0	115 bp	1.0	63.1
trnL-UAG-ccsA_p5	GTAGACACGCTGCTCTTAGG	AGGAGTAGGATCCATTGTGTCT	55.0	45.5	57.8	58.0	114 bp	1.0	63.1