Hotspot

psbT-psbN

LSC · 78888-78951 · 64 bp

Back to Cautleya

Pi 0.125

MarkerSeek score 87.50

Primer available yes

Label psbT-psbN

Feature Metadata

label_name: psbT-psbN
feature_type: igs
region: LSC
length_bp: 64
variable_sites: 8
pi: 0.125
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 87.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbT-psbN_p1	TTTTCGAGAACCGCCTAAGG	ATCCATTCGAAGAACACGGG	50.0	50.0	57.9	58.0	133 bp	1.0	83.9
psbT-psbN_p2	ACCGCCTAAGGTTCCAACTA	ATCCATTCGAAGAACACGGG	50.0	50.0	58.4	58.0	124 bp	1.0	82.2
psbT-psbN_p3	TTTTCGAGAACCGCCTAAGG	TTCGAAGAACACGGGGACTA	50.0	50.0	57.9	58.4	128 bp	1.0	81.2
psbT-psbN_p4	TTTTCGAGAACCGCCTAAGG	GGGTACGCCTTATATACCGC	50.0	55.0	57.9	57.7	182 bp	1.0	80.5
psbT-psbN_p5	TTTTCGAGAACCGCCTAAGG	GCAACCCTCTCGACAACTAA	50.0	50.0	57.9	57.5	156 bp	1.0	79.8