Hotspot

psbE-petL

LSC · 68907-70191 · 1285 bp

Back to Cautleya

Pi 0.00472

MarkerSeek score 62.60

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1285
variable_sites: 6
pi: 0.00472
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	AAGCAGCCAGTAGAAAACCG	50.0	50.0	57.9	58.5	1417-1432 bp	1.0	77.0
psbE-petL_p2	CAACCCGCAATGAATAGGGA	GCAGCCAGTAGAAAACCGAA	50.0	50.0	57.9	58.5	1415-1430 bp	1.0	76.9
psbE-petL_p3	ACTGACGAATAACCAACCCG	AAGCAGCCAGTAGAAAACCG	50.0	50.0	57.9	58.5	1430-1445 bp	1.0	76.6
psbE-petL_p4	ACTGACGAATAACCAACCCG	GCAGCCAGTAGAAAACCGAA	50.0	50.0	57.9	58.5	1428-1443 bp	1.0	76.6
psbE-petL_p5	ACGAATAACCAACCCGCAAT	AAGCAGCCAGTAGAAAACCG	45.0	50.0	58.2	58.5	1426-1441 bp	1.0	76.0