Hotspot

trnC-GCA-petN

LSC · 29513-30392 · 880 bp

Back to Cautleya

Pi 0.0024

MarkerSeek score 61.80

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 880
variable_sites: 2
pi: 0.0024
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	ATTTGGATCGGATTTGGCGA	AGTCCACTCCTTCCCCATAC	45.0	55.0	57.9	58.1	1008-1052 bp	1.0	82.4
trnC-GCA-petN_p2	ATTTGGATCGGATTTGGCGA	GTCCACTCCTTCCCCATACT	45.0	55.0	57.9	58.1	1007-1051 bp	1.0	82.4
trnC-GCA-petN_p3	ATTTGGATCGGATTTGGCGA	AAAGTCCACTCCTTCCCCAT	45.0	50.0	57.9	58.2	1010-1054 bp	1.0	81.4
trnC-GCA-petN_p4	ATTTGGATCGGATTTGGCGA	CCTCCCTAAAGTCCACTCCT	45.0	55.0	57.9	57.7	1017-1061 bp	1.0	81.2
trnC-GCA-petN_p5	ATTTGGATCGGATTTGGCGA	TACCCCTCCCTAAAGTCCAC	45.0	55.0	57.9	57.7	1021-1065 bp	1.0	81.2