Hotspot

rpoB-trnC-GCA

LSC · 28242-29441 · 1200 bp

Back to Cautleya

Pi 0.00336
MarkerSeek score 61.60
Primer available yes
Label rpoB-trnC-GCA

Feature Metadata

label_name
rpoB-trnC-GCA
feature_type
igs
region
LSC
length_bp
1200
variable_sites
4
pi
0.00336
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoB-trnC-GCA_p1 AATTCTTCCGTCAAGCCCTC AAAGGATTTGCAGTCCCCTG 50.0 50.0 57.9 58.1 1344-1350 bp 1.0 79.4
rpoB-trnC-GCA_p2 AATTCTTCCGTCAAGCCCTC GCGACACCCAGATTTGAACT 50.0 50.0 57.9 58.5 1370-1376 bp 1.0 76.1
rpoB-trnC-GCA_p3 AATTCTTCCGTCAAGCCCTC CCTTACCACTTGGCCATGTC 50.0 55.0 57.9 58.5 1324-1330 bp 1.0 75.7
rpoB-trnC-GCA_p4 AAATTCTTCCGTCAAGCCCT AAAGGATTTGCAGTCCCCTG 45.0 50.0 57.4 58.1 1345-1351 bp 1.0 75.6
rpoB-trnC-GCA_p5 CTTCCGTCAAGCCCTCATTA AAAGGATTTGCAGTCCCCTG 50.0 50.0 57.3 58.1 1340-1346 bp 1.0 74.8