Hotspot

atpF-atpH

LSC · 13647-14158 · 512 bp

Back to Cautleya

Pi 0.00783

MarkerSeek score 61.60

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 512
variable_sites: 4
pi: 0.00783
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	AGTGGCCCAAGTAAACGAAA	TGGTTGTGGCATTAGCACTT	45.0	45.0	57.6	58.0	596 bp	1.0	77.8
atpF-atpH_p2	CAGTGGCCCAAGTAAACGAA	TGGTTGTGGCATTAGCACTT	50.0	45.0	58.4	58.0	597 bp	1.0	77.4
atpF-atpH_p3	TGGCCCAAGTAAACGAAAGA	TGGTTGTGGCATTAGCACTT	45.0	45.0	57.4	58.0	594 bp	1.0	75.5
atpF-atpH_p4	GTGGCCCAAGTAAACGAAAG	TGGTTGTGGCATTAGCACTT	50.0	45.0	57.3	58.0	595 bp	1.0	75.0
atpF-atpH_p5	TTAAACCCGAAACTTCCGGC	TGGTTGTGGCATTAGCACTT	50.0	45.0	58.8	58.0	624 bp	1.0	74.5