Hotspot

ycf1

SSC · 110462-116080 · 5619 bp

Back to Caulophyllum

Pi 0.374

MarkerSeek score 74.10

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5619
variable_sites: 1756
pi: 0.374
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.83
markerseek_score: 74.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AGTCCTTTCCTCATTTGCGG	TTTCGGGGAGAACCAAAAGG	50.0	50.0	58.2	58.0	1068 bp	0.5	58.5
ycf1_p2	GTCCTTTCCTCATTTGCGGA	TTTCGGGGAGAACCAAAAGG	50.0	50.0	58.2	58.0	1067 bp	0.5	58.5
ycf1_p3	AGTCCTTTCCTCATTTGCGG	GTGCCAAGGTTTCAGACAGA	50.0	50.0	58.2	58.0	949 bp	0.5	58.3
ycf1_p4	AGTCCTTTCCTCATTTGCGG	TCTGTGCCAAGGTTTCAGAC	50.0	50.0	58.2	58.0	952 bp	0.5	58.3
ycf1_p5	GTCCTTTCCTCATTTGCGGA	GTGCCAAGGTTTCAGACAGA	50.0	50.0	58.2	58.0	948 bp	0.5	58.3