Hotspot

rpl32

SSC · 127397-127561 · 165 bp

Back to Caulophyllum

Pi 0.474

MarkerSeek score 77.30

Primer available yes

Label rpl32

Feature Metadata

label_name: rpl32
feature_type: gene
region: SSC
length_bp: 165
variable_sites: 74
pi: 0.474
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 77.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32_p1	CCCATCCCGCAAAAACCTAT	CGCGGGTAAGGATTCTGAAA	50.0	50.0	58.2	58.0	824 bp	0.5	58.1
rpl32_p2	ACCCCACTCCCATTTTTGAG	CGCGGGTAAGGATTCTGAAA	50.0	50.0	57.7	58.0	1303 bp	0.5	57.4
rpl32_p3	CCCATCCCGCAAAAACCTAT	GACGAAAATCGCCGGAGATA	50.0	50.0	58.2	57.9	719 bp	0.5	57.2
rpl32_p4	CCCATCCCGCAAAAACCTAT	ACGAAAATCGCCGGAGATAC	50.0	50.0	58.2	58.2	718 bp	0.5	57.0
rpl32_p5	CCCATCCCGCAAAAACCTAT	CGAAAATCGCCGGAGATACA	50.0	50.0	58.2	58.2	717 bp	0.5	57.0