Hotspot

ndhH

SSC · 116875-118056 · 1182 bp

Back to Caulophyllum

Pi 0.477

MarkerSeek score 81.30

Primer available yes

Label ndhH

Feature Metadata

label_name: ndhH
feature_type: gene
region: SSC
length_bp: 1182
variable_sites: 489
pi: 0.477
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 81.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH_p1	TCAGAGGGGTTTGCGAAAAA	CCCGAGACTGAATAAACGCA	45.0	50.0	57.9	58.0	1749 bp	0.5	59.5
ndhH_p2	TCAGAGGGGTTTGCGAAAAA	AGTGTCCTAACGAATCGCAC	45.0	50.0	57.9	58.0	2019 bp	0.5	59.5
ndhH_p3	TCAGAGGGGTTTGCGAAAAA	TTAGTTCATCGCCCCGAATG	45.0	50.0	57.9	58.1	2121 bp	0.5	59.1
ndhH_p4	TCAGAGGGGTTTGCGAAAAA	TGAAGAATTCCCAAAGGGCC	45.0	50.0	57.9	58.1	1667 bp	0.5	59.0
ndhH_p5	TATCTCAGAGGGGTTTGCGA	CCCGAGACTGAATAAACGCA	50.0	50.0	58.1	58.0	1753 bp	0.5	58.8