Hotspot

ccsA

SSC · 125235-126206 · 972 bp

Back to Caulophyllum

Pi 0.469

MarkerSeek score 79.00

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 972
variable_sites: 358
pi: 0.469
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.78
markerseek_score: 79.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	TCTGGACCACGAGAACTCTT	TTCTCTGCCGGGACTAAGAA	50.0	50.0	58.0	58.1	2124 bp	0.5	59.4
ccsA_p2	TTCTGGACCACGAGAACTCT	TTCTCTGCCGGGACTAAGAA	50.0	50.0	58.0	58.1	2125 bp	0.5	59.4
ccsA_p3	TCTGGACCACGAGAACTCTT	GAACAGAACTCTCCGGTTCC	50.0	55.0	58.0	57.9	2107 bp	0.5	59.3
ccsA_p4	TTCTGGACCACGAGAACTCT	GAACAGAACTCTCCGGTTCC	50.0	55.0	58.0	57.9	2108 bp	0.5	59.3
ccsA_p5	TCTGGACCACGAGAACTCTT	TAGACACGCTGCTCTTAGGA	50.0	50.0	58.0	57.9	1848 bp	0.5	59.0