Hotspot

trnL-UAG-rpl32

SSC · 126386-127396 · 1011 bp

Back to Caulophyllum

Pi 0.457

MarkerSeek score 81.60

Primer available yes

Label trnL-UAG-rpl32

Feature Metadata

label_name: trnL-UAG-rpl32
feature_type: igs
region: SSC
length_bp: 1011
variable_sites: 407
pi: 0.457
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 81.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAG-rpl32_p1	AAGTAGCAAAGCCTTGGGTC	CGCGGGTAAGGATTCTGAAA	50.0	50.0	58.1	58.0	2101 bp	0.5	59.1
trnL-UAG-rpl32_p2	GAAGTAGCAAAGCCTTGGGT	CGCGGGTAAGGATTCTGAAA	50.0	50.0	58.1	58.0	2102 bp	0.5	59.1
trnL-UAG-rpl32_p3	TGAAGTAGCAAAGCCTTGGG	CGCGGGTAAGGATTCTGAAA	50.0	50.0	58.1	58.0	2103 bp	0.5	59.1
trnL-UAG-rpl32_p4	TCCTAAGAGCAGCGTGTCTA	CGCGGGTAAGGATTCTGAAA	50.0	50.0	57.9	58.0	1618 bp	0.5	58.9
trnL-UAG-rpl32_p5	AAGTAGCAAAGCCTTGGGTC	TCATTTTTGCATGGCGGTTC	50.0	45.0	58.1	57.9	1708 bp	0.5	58.7