Hotspot

ndhD-ccsA

SSC · 124684-125234 · 551 bp

Back to Caulophyllum

Pi 0.449

MarkerSeek score 71.70

Primer available yes

Label ndhD-ccsA

Feature Metadata

label_name: ndhD-ccsA
feature_type: igs
region: SSC
length_bp: 551
variable_sites: 128
pi: 0.449
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.52
markerseek_score: 71.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-ccsA_p1	TCTGGACCACGAGAACTCTT	GCGAGATCCAAGACGTGAAT	50.0	50.0	58.0	58.1	1126 bp	0.5	59.4
ndhD-ccsA_p2	TTCTGGACCACGAGAACTCT	GCGAGATCCAAGACGTGAAT	50.0	50.0	58.0	58.1	1127 bp	0.5	59.4
ndhD-ccsA_p3	TCTGGACCACGAGAACTCTT	ACCCAAGGCTTTGCTACTTC	50.0	50.0	58.0	58.1	1364 bp	0.5	59.3
ndhD-ccsA_p4	TCTGGACCACGAGAACTCTT	GACCCAAGGCTTTGCTACTT	50.0	50.0	58.0	58.1	1365 bp	0.5	59.3
ndhD-ccsA_p5	TTCTGGACCACGAGAACTCT	ACCCAAGGCTTTGCTACTTC	50.0	50.0	58.0	58.1	1365 bp	0.5	59.3