Hotspot

ndhI-ndhG

SSC · 120853-121400 · 548 bp

Back to Caulophyllum

Pi 0.424

MarkerSeek score 81.40

Primer available yes

Label ndhI-ndhG

Feature Metadata

label_name: ndhI-ndhG
feature_type: igs
region: SSC
length_bp: 548
variable_sites: 211
pi: 0.424
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 81.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI-ndhG_p1	TGGTCAACAAACAGTCCGAG	AATCCCAAGTTGTTGACCCC	50.0	50.0	58.1	58.0	1506 bp	0.5	59.5
ndhI-ndhG_p2	AGTCTGTGTTCGTGTATGCC	AATCCCAAGTTGTTGACCCC	50.0	50.0	57.9	58.0	1335 bp	0.5	59.3
ndhI-ndhG_p3	TGCCCTATTGATCTACCCGT	AATCCCAAGTTGTTGACCCC	50.0	50.0	57.9	58.0	1319 bp	0.5	59.1
ndhI-ndhG_p4	TGGTCAACAAACAGTCCGAG	AATCCCGTACCAGGACGTAT	50.0	50.0	58.1	57.9	1437 bp	0.5	59.0
ndhI-ndhG_p5	TGGTCAACAAACAGTCCGAG	ATAATCCCGTACCAGGACGT	50.0	50.0	58.1	57.9	1439 bp	0.5	59.0