Hotspot

ccsA-ndhD

SSC · 113124-113399 · 276 bp

Back to Cattleya

Pi 0.04

MarkerSeek score 61.40

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 276
variable_sites: 11
pi: 0.04
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	ATGCAGCTCTTTTATGCGGA	TTCTCGATGGCTTCTCTTGC	45.0	50.0	58.0	58.0	1162-1163 bp	1.0	80.6
ccsA-ndhD_p2	TGCAGCTCTTTTATGCGGAT	TTCTCGATGGCTTCTCTTGC	45.0	50.0	58.0	58.0	1161-1162 bp	1.0	80.6
ccsA-ndhD_p3	ATGCAGCTCTTTTATGCGGA	CTCGATGGCTTCTCTTGCAT	45.0	50.0	58.0	58.0	1160-1161 bp	1.0	80.3
ccsA-ndhD_p4	TGCAGCTCTTTTATGCGGAT	CTCGATGGCTTCTCTTGCAT	45.0	50.0	58.0	58.0	1159-1160 bp	1.0	80.3
ccsA-ndhD_p5	TGCAGCTCTTTTATGCGGAT	GGTTCTATAACTGGCACGGG	45.0	55.0	58.0	58.1	1343-1344 bp	1.0	80.2