Hotspot

trnN-GUU-rpl32

SSC · 110129-111084 · 956 bp

Back to Cattleya

Pi 0.0379

MarkerSeek score 66.80

Primer available yes

Label trnN-GUU-rpl32

Feature Metadata

label_name: trnN-GUU-rpl32
feature_type: igs
region: SSC
length_bp: 956
variable_sites: 36
pi: 0.0379
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 66.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-rpl32_p1	GGCAGAATAGCAGAGCAAGT	GATTGTCCGGTGGAAATCGA	50.0	50.0	58.0	58.0	1491-1570 bp	1.0	81.8
trnN-GUU-rpl32_p2	AAAGCGAGCCCTTTCATTCA	GATTGTCCGGTGGAAATCGA	45.0	50.0	58.1	58.0	1209-1288 bp	1.0	81.8
trnN-GUU-rpl32_p3	AAGCGAGCCCTTTCATTCAA	GATTGTCCGGTGGAAATCGA	45.0	50.0	58.1	58.0	1208-1287 bp	1.0	81.8
trnN-GUU-rpl32_p4	TCAAAGCGAGCCCTTTCATT	GATTGTCCGGTGGAAATCGA	45.0	50.0	58.1	58.0	1211-1290 bp	1.0	81.8
trnN-GUU-rpl32_p5	GTAACCTGGGCTGGAATCAG	GATTGTCCGGTGGAAATCGA	55.0	50.0	58.0	58.0	1556-1635 bp	1.0	81.6