Hotspot

petB-petD

LSC · 77725-78716 · 992 bp

Back to Cattleya

Pi 0.00404

MarkerSeek score 61.20

Primer available yes

Label petB-petD

Feature Metadata

label_name: petB-petD
feature_type: igs
region: LSC
length_bp: 992
variable_sites: 4
pi: 0.00404
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB-petD_p1	ATTGCCTCTTCTTACTGCCG	GATCCATCCCCCAAAGGAAC	50.0	55.0	58.0	57.9	1097-1098 bp	1.0	79.5
petB-petD_p2	AAGCAAGGTATTTCGGGTCC	GATCCATCCCCCAAAGGAAC	50.0	55.0	57.9	57.9	1045-1046 bp	1.0	78.8
petB-petD_p3	GCAAGGTATTTCGGGTCCTT	GATCCATCCCCCAAAGGAAC	50.0	55.0	57.9	57.9	1043-1044 bp	1.0	78.8
petB-petD_p4	ATTGCCTCTTCTTACTGCCG	ATGTCCCATCCCTTTAGCCA	50.0	50.0	58.0	58.4	1184-1185 bp	1.0	77.0
petB-petD_p5	GCAAGGTATTTCGGGTCCTT	ATGTCCCATCCCTTTAGCCA	50.0	50.0	57.9	58.4	1130-1131 bp	1.0	76.2