Hotspot

clpP-psbB

LSC · 72961-73920 · 960 bp

Back to Cattleya

Pi 0.0159
MarkerSeek score 63.70
Primer available yes
Label clpP-psbB

Feature Metadata

label_name
clpP-psbB
feature_type
igs
region
LSC
length_bp
960
variable_sites
15
pi
0.0159
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
63.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
clpP-psbB_p1 CGCACTATACGTCAACCCAA GGCCCAACCAGAAACTAGAG 50.0 55.0 58.0 57.9 1138-1142 bp 1.0 81.2
clpP-psbB_p2 TATACGTCAACCCAAGCTGC GGCCCAACCAGAAACTAGAG 50.0 55.0 58.0 57.9 1133-1137 bp 1.0 81.0
clpP-psbB_p3 CGCACTATACGTCAACCCAA CGATACCAAGGCAAACCCAT 50.0 50.0 58.0 58.2 1056-1060 bp 1.0 80.4
clpP-psbB_p4 TATACGTCAACCCAAGCTGC CGATACCAAGGCAAACCCAT 50.0 50.0 58.0 58.2 1051-1055 bp 1.0 80.2
clpP-psbB_p5 CTTTTGGAACACCAATGGGC GGCCCAACCAGAAACTAGAG 50.0 55.0 57.8 57.9 1084-1088 bp 1.0 79.9